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M94A2772.TXT
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1994-10-25
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Document 2772
DOCN M94A2772
TI Use of agglutination test for the determination of salivary HIV-1
antibodies.
DT 9412
AU Castillo Torres NP; Barriga AG; Hospital Infectologia Centro Medico La
Raza, Mexico, D.F.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):228 (abstract no. PB0343). Unique
Identifier : AIDSLINE ICA10/94369803
AB OBJECTIVE: To demonstrate that saliva samples collected with a special
device (Omni-Sal) can be tested accurately with a gelatin agglutination
test (Serodia) for antibodies to HIV-1 in a clinical setting. METHODS:
Matched serum and saliva specimens were collected from 150 persons
including 75 known HIV-1 positive patients, 51 having diverse
infectious, metabolic, hematologic and autoimmune diseases and 24
healthy individuals. All saliva specimens were obtained using the
Omni-Sal collection device. All specimens were assayed with the Serodia
HIV-1 test using a-minor modification of the assay procedure. RESULTS:
Serum and saliva results were in 96.7% agreement when
minor-modifications were performed to the Serodia procedure for serum,
in any case there were false negative results with saliva or serum
samples. CONCLUSIONS: Saliva obtained with the Omni-Sal device can be
tested for HIV-1 antibodies using the serodia agglutination test
following minor-modifications of the assay procedure recommended for
serum samples.
DE *Agglutination Tests Autoimmune Diseases/IMMUNOLOGY Comparative Study
Evaluation Studies False Negative Reactions Human HIV
Antibodies/*ANALYSIS/BLOOD HIV Infections/*DIAGNOSIS HIV-1/*IMMUNOLOGY
Infection/IMMUNOLOGY Sensitivity and Specificity Specimen
Handling/*INSTRUMENTATION MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).